Inclusion Criteria:

1.Healthy as determined by a responsible physician, based on a medical evaluation including medical history, physical examination, laboratory tests and cardiac monitoring. A subject with a clinical abnormality or laboratory parameters outside the reference range for the population being studied may be included only if the Investigator and the GSK Medical Monitor agree that the finding is unlikely to introduce additional risk factors and will not interfere with the study procedures.
2. Male or female aged between 18 and 55 years inclusive.
3. A female subject is eligible to participate if she is of non-childbearing potential:
4. Non-childbearing potential is defined as pre-menopausal females with a documented tubal ligation or hysterectomy; or postmenopausal defined as 12 months of spontaneous amenorrhoea [in questionable cases a blood sample with simultaneous
follicle stimulating hormone (FSH) > 40 MlU/ml and oestradiol < 40 pg/ml (<140 pmol/L) is confirmatory.]1
5. Male subjects must agree to use one of the contraception methods listed in Section 8.1. This criterion must be followed from the time of the first dose of study medication until 5 x terminal half-lives after the last dose.
6. Body weight > 50 kg and BMI within the range 18.5 – 29.9 kg/m2 (inclusive).
7. Capable of giving written informed consent, which includes compliance with the requirements and restrictions listed in the consent form. The written informed consent form should be signed and dated.
8. QTcB or QTcF < 450 msec; or QTc < 480 msec in subjects with Bundle Branch Block. A 12-lead ECG at pre-study screening, which in the opinion of the Principal Investigator or physician designee has no abnormalities that will compromise safety in this study. The specific inclusion criterion with regard to QT interval (either QTcb or QTcf, machine or manual over-read, males or females) is that QTc should be less than 450msec (based on single or average QTc of triplicate ECGs obtained over a brief recording period).
9. No abnormality on relevant clinical chemistry or haematology examination at the pre-study medical examination. Subjects with laboratory parameters outside the reference range for this age group will only be included if the principal Investigator or designee considers that such findings will not introduce additional risk factors. Consultation with the GSK medical monitor is required before such subjects may be included. In any case, liver function tests (including bilirubin, ALT, AST or alkaline phosphatase) and markers of renal function (including urea and creatinine) must be strictly within the normal range at screening.
10. Normal physical examination (providing no evidence of clinically active disease or physical or mental impairment). A subject with a clinical abnormality may be included only if the Principal Investigator or physician designee considers that the abnormality will not introduce additional risk factors and will not interfere with study procedures. Consultation with the GSK medical monitor is required before such subjects may be included.
11. A 24-hour Holter at pre-study screening, which in the opinion of the Principal investigator or physician designee has no abnormalities that will compromise safety in this study. If a rhythm abnormality is detected, the subject may be included in the study only if the Principle Investigator or physician designee considers that the abnormality will not introduce additional risk factors and will not interfere with study procedures. Consultation with Principal Investigator, GSK medical monitor
and, if required, a specialist cardiologist will occur before such a subject may be included.


Exclusion Criteria:

1.The subject has a positive pre-study drug/alcohol screen. A minimum list of drugs
that will be screened for include amphetamines, barbiturates, cocaine, opiates,
cannabinoids and benzodiazepines.
2. A positive pre-study Hepatitis B surface antigen or positive Hepatitis C antibody
result within 3 months of screening.
3. A positive test for HIV antibody.
4. Subjects with any risk factors for tuberculosis that include: (i) a history of either
congenital or acquired immunosuppression (e.g. HIV), (ii) a history of any disorder
that may be a function of altered immunomodulation (e.g. autoimmune disease), (iii)
a history of any clinical condition or surgical procedure that places them at higher
risk of TB (e.g. diabetes, sarcoidosis, gastrectomy and vagotomy, malignancy,
leukaemia), (iv) a history of mycobacterial infection, including TB, (v) recent close
contact (within the previous 48 months) with an individual known to be infected with
TB, (vi) recent immigrants or residents (within the previous 48 months) from
countries where TB is considered to be of high-prevalence. For the purposes of TB
screening amongst applicants for UK visas, high prevalence countries include
Bangladesh, Ghana, Burkina Faso, Cote d’Ivoire, Togo, Niger, Kenya, Eritrea,
Somalia, Pakistan, Sudan, Tanzania, Thailand, Cambodia and Laos
(http://www.ukvisasgov.uk/en/howtoapply/tbscreening).
5. History of regular excessive alcohol consumption within 6 months of the study defined as:
6. an average weekly intake of greater than 21 units or an average daily intake of
greater than 3 units (males), or defined as an average weekly intake of greater than
14 units or an average daily intake of greater than 2 units (females). One unit is
equivalent to a half-pint (220mL) of beer or 1 (25ml) measure of spirits or 1 glass (125ml) of wine.
7. The subject has participated in a clinical trial and has received an investigational
product within the following time period prior to the first dosing day in the current
study: 90 days, 5 half-lives or twice the duration of the biological effect of the investigational product (whichever is longer).
8. Exposure to more than four new chemical entities within 12 months prior to the first dosing day.
9. Use of prescription or non-prescription drugs, including vitamins, herbal and dietary supplements (including St John’s Wort) within 7 days (or 14 days if the drug is a potential enzyme inducer) or 5 half-lives (whichever is longer) prior to the first dose
of study medication, unless in the opinion of the Investigator and GSK Medical Monitor that the medication will not interfere with the study procedures or compromise subject safety.ONFIDENTIAL HH2007/00234/
10. History of sensitivity to any of the study medications, or components thereof, or a history of drug or other allergy that, in the opinion of the investigator or GSK Medical Monitor, contraindicates their participation.
11. Where participation in the study would result in donation of blood or blood products in excess of 500 mL within a 90 day period before the screening visit.
12. Pregnant females as determined by positive serum hCG test at screening or urine hCG test prior to dosing.
13. Lactating females.
14. Females of child-bearing potential
15. Females receiving Hormone Replacement Theory
16. Unwillingness or inability to follow the procedures outlined in the protocol.
17. Urinary cotinine levels indicative of smoking or a history of regular use of tobaccoor nicotine-containing products within 6 months prior to screening.
18. Has a resting systolic blood pressure which is outside the range of 90 to 140 mmHg, diastolic blood pressure is outside the range of 50 to 90 mmHg or heart rate is outside the range 90 to 40 bpm.

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• GSK Investigational Site
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